CTAB-Mediated Purification of PCR Products

CTAB-mediated purification of PCR products.

Gel Purification of PCR Products. Fatemeh Mehrpooyan*

An efficient method for purification of PCR products for sequencing.

A high-throughput DNA sequencing method that generated high quality data was developed. A frame fashioned from a standard agarose gel combined with 0.1%-0.2% low-melting point (LMP) agarose gel was used to isolate the PCR product of interest. Collected PCR products were centrifuged without any reagents and the supernatants were directly used for a sequencing reaction. This method is simple and ...

Distinct combination of purification methods dramatically improves cohesive-end subcloning of PCR products.

For most purposes, cloning protocols based on the polymerase chain reaction (PCR) using either the template-independent terminal transferase activity of Taq DNA polymerase in TA cloning schemes (8,9) or blunt-endbased procedures involving Pwo or Pfu DNA polymerase or polished Taq-generated PCR products are quite satisfactory. Nevertheless, both techniques have been proven less straightforward t...

Convenient single-step, one tube purification of PCR products for direct sequencing.

Structural alterations of the apolipoprotein E (apo E) are known to influence the lipid metabolism. The three most common isoproteins Arg 158) are encoded by three co-dominant alleles (e2, el, e4). Phenotyping is cumbersome and, therefore, several methods for apo E genotyping have been developed. Of these, PCR-RFLP (restriction fragment length polymorphism) is currently most rapid and cost-effe...